1. SCAR marker of Dabailai orange leaf ball and its application in assisted selection breeding
Application (Patent) No. 9
Abstract: The present invention discloses a SCAR marker of Chinese cabbage orange leaf ball and the application of this SCAR marker for assisting selective breeding. The assisted selection breeding method was based on the genomic DNA of color Chinese cabbage and common white Chinese cabbage and its second generation segregating population as the template. RAPD markers were used to identify the RAPD markers linked to the color orange cabbage leaves by repeated experiments. Successfully transformed into a specific SCAR marker. Linkage analysis found that the linkage distance between the marker and the orange leaf ball was 3.7 cm. Using this SCAR marker, molecular assisted selection breeding of orange leaf ball traits in Chinese cabbage can be performed.
2. A Chinese cabbage variety identification and seed purity test method using SCAR markers
Application (Patent) No.4
Abstract : The present invention discloses a method for identifying Chinese cabbage varieties and testing seed purity using SCAR markers. The genomic DNA of the new Chinese cabbage variety Guanchun and its parents was used as a template. RAPD fingerprints of the hybrid “Guanchun†hybrid and its parents were established by RAPD analysis, and a female was successfully obtained. This specific SCAR marker and a hybrid-specific SCAR marker can be used to identify the cultivar of the "Guanchun" hybrid and to test the purity of the seed according to the cultivar fingerprint and specific markers. Through the identification of 134 random populations of crown and spring, the identification results were consistent with the field identification.
3. The cloning method of the Tianbailai chitinase CHB4 gene
Application (Patent) No.1
Abstract : The Chinese cabbage chitinase CHB4 gene and its cloning method are characterized by the use of a known chitinase gene sequence of Brassicaceae and the cloning of specific DNA fragments from the genomic DNA of Chinese cabbage through comparative design of primers. And the 5′-end sequence fragment; the 3′-end sequence fragment was obtained through RACE reaction using leaf RNA of the treatment plant induced by salicylic acid and with the highest enzyme activity as a template, based on the cloning of the chitinase gene fragment of Chinese cabbage. The redesigned primers amplified the full-length 1517 bp of the chitinase CHB4 gene from the genomic DNA by PCR. The invention further uses the bioengineering technology to transfer the exogenous chitinase gene into various plants, so as to improve the antifungal ability of plants and provide a basis for breeding resistant varieties.
4. A Breeding Method of Chinese Cabbage Hybrids
Application (Patent) No. 9
Abstract : The invention discloses a Chinese cabbage hybrid breeding method. The method utilizes the rape radish cytoplasmic male sterility material RC97-1 as a female parent and the Chinese cabbage inbred line 1138 as a male parent, through the interspecies Crossbreeding to obtain the first-generation seeds, selecting and retaining the excellent single plants that are similar to those of the parent to be used as the next round of backcross females; after 7 consecutive generations of backcrossing, and through economic trait selection and resistance For disease identification, the sterility of the Chinese cabbage cytoplasmic male sterile line germplasm material RC7 with stable sterility, sterile plant rate and sterility of 100%, and economic traits and corresponding maintenance lines were selected; The Chinese cabbage cytoplasm male sterile line RC7 was a female parent. Hybridization with the Chinese cabbage inbred line 0351207 resulted in a Chinese cabbage hybrid. The Chinese cabbage hybrid generation RC7×03S1207 (Jinqiu 90), which was selected by this method, has the characteristics of disease resistance, high quality, high yield, stable yield, and strong adaptability.
5. Breeding method of Chinese cabbage hybrids
Application (Patent) No.4
Abstract: The present invention discloses a Chinese cabbage hybrid breeding method, characterized in that the method uses female sterility male sterile material RC97-1 of Brassica napus as female parent and male parent Chinese inbred line 1138 as male parent. The seeds of the first generation were obtained through cross-breeding, and the fine individuals with the same performance as those of the male parent were selected as the backcross female parent for the next round; Character selection, disease resistance identification, selection of sterility stable, sterile plant rate and sterility of 100%, the economic traits and the corresponding maintainer line of Chinese cabbage cytoplasmic male sterile line germplasm material RC7; Then Chinese cabbage hybrids can be obtained by using parental Chinese cabbage cytoplasmic male sterile line RC7 as female parent and Chinese cabbage inbred line 045245 as male parent. The Chinese cabbage hybrid generation Jinqiu 70 (RC7×04S245) obtained by this method has the characteristics of early ripening, uniform and consistent plant type, strong disease resistance, wide adaptability, high quality, high yield, and stable yield.
6. A Breeding Method for the Germplasm Resources of Dalla Cytoplasmic Male Sterile Lines
Application (Patent) No.X
Abstract : The invention discloses a method for breeding germplasm material of cytoplasmic male sterile line of Chinese cabbage. The method uses female sterilized material RC97-1 of Brassica napus as female parent and Chinese cabbage inbred line. 1138 is a male parent. A cross-breeding seed is obtained through inter-species hybridization. The selected elite leaves that have similar performance to their male parents are selected as the backcross female parent for the next round; , After selection of economic traits and identification of disease resistance, the sterility was stable, the sterile plant rate and sterility reached 100%, and the economic traits and the corresponding maintainer line were completely consistent with the Chinese cabbage cytoplasmic male sterile line. Material RC7. The germplasm material RC7 of the present invention can not only maintain the sterility of Ogura radish cytoplasmic CMS male sterile line, but also overcome its yellowing, growth retardation, nectary degeneration and coordination. For the maintenance of other defects. It can attract bees and other insects to normally collect pollination and pollination, has good combining ability, and has comprehensive resistance to disease and has high value of utilization.
7. Chinese cabbage leafhopper double-incidence breeding method
Application (Patent) No. 5
Abstract: For Chinese cabbage harvested in autumn, the conventional method of harvesting is to uproot it, cut the leaf ball, and treat the rest of the leaves and roots as feed or field waste. It does not know that this is a waste of resources. . The invention provides a Chinese cabbage leafhopper double-receiving cultivation method. By timely cutting the leaf ball and retaining the leaves and roots, two additional high-quality cabbage seeds can be added on the basis of the original plant, thereby greatly increasing the size of the cabbage leaf. Cabbage planting benefits.
8. A Breeding Method for Cytoplasmic Male Sterility Line of Chinese Cabbage
Application (Patent) No.4
Abstract: The present invention belongs to the technical field of crucifer vegetable breeding. Specifically, the invention belongs to the invention of a Chinese cabbage cytoplasmic male sterile line breeding method. The technical feature of the present invention is that natural male sterile Brassica juncea strains are selected and maintained by the elite Chinese cabbage DH line 63-5, and genetic stability is achieved by distant hybridization, embryo rescue and continuous backcrossing and molecular marker assisted selection. The cytoplasmic male sterile line of Chinese cabbage was propagated and the Chinese sterile line CMS63-5, a cytoplasmic male sterile line of Chinese cabbage available for production and utilization, was obtained. The cytoplasmic sterile type of the Chinese cabbage sterile line is the same as that of 6-102A, and it is not related to cytoplasmic males such as pol CMS, Ogu CMS, tour CMS and nap CMS. The type of education is not the same, is a new type of cytoplasmic male sterility, the sterility and sterile plant rate of the sterile line are 100%, and is not affected by the environment and genetic background. The Chinese cabbage cytoplasmic male sterile line of the invention can be used for hybrid breeding of vegetables.
9. Method for culturing primary three systems of Chinese cabbage
Application (Patent) No. 2
Abstract : The invention discloses a method for cultivating primary three systems of Chinese cabbage, which belongs to the field of cytogenetics theory and breeding technology. The method includes (1) free microspore culture; (2) establishment of microspores individual lines; (3) screening of 2n+1 (trisomic) plants; (4) identification of trisomy types; Determination of sexual characteristics. The present invention is characterized in that the isolated microspores of tetraploid Chinese cabbage are cultured in vitro using plant tissue and cell culture techniques, and then the primary trisomy is screened and identified directly from the microspores obtained in the culture. Using the method of the present invention to create a primary three-body, high efficiency, short time, compared to the conventional creation method, to create a set of primary three-body, generally can be shortened by 2-3 years. A set of Chinese cabbage primary trisomics created by the invention can be widely used for gene mapping, genetic effects research, chromosome engineering breeding and molecular engineering breeding of Chinese cabbage.
10. Huaiyang Cabbage Cultivation Technology
Application (Patent) No. 5
Abstract: The invention relates to a cultivation technique of Huaiyang Chinese cabbage, which is suitable for large-scale cultivation of vegetable farmers. In order to overcome the shortage of Chinese cabbage in the spring, summer and winter markets, the present invention adopts four-year planting and scientific management of the shed to achieve a balanced supply of fresh vegetables for the four seasons and to regulate the demand for vegetables in urban and rural areas.
11. Chinese Cabbage Health Drink
Application (Patent) No. 9
Abstract: Chinese cabbage health drinks belong to food and drink. The invention provides a liquid Chinese cabbage health drink with Chinese cabbage as a main raw material. The present invention is characterized in that: 20 to 80% of Chinese cabbage, 5 to 60% of apple juice, 1 to 15% of white granulated sugar, 1 to 15% of fructose syrup, 1 to 5% of 9-degree rice vinegar, 0.01 to 0.10% of citric acid, and vitamins C0.01 ~ 0.03%; food coloring; edible flavor amount; pure water to 100%.
12, a color Chinese cabbage breeding method
Application (Patent) No. 9
Abstract : The invention discloses a method for breeding color Chinese cabbage, which is selected by using the self-incompatibility line 01S941 of female germplasm material and the self-incompatibility line 01S915 of paternal germplasm. Its outer leaves are dark green, 2 to 3 leaves of the outer layer of the bulb are green, the inner leaf color is golden yellow, and the leaf color becomes slightly red in the sun; the plant height is 40cm, the plant width is 70cm; the leaf ball is folded and held high Headball type, ball index 1.28; single ball net weight 2.5 ~ 3kg; mid-maturity hybrids, reproductive period of about 80 ~ 85d; seed production, the parent and female parent sowing at the same time; the ratio of the parent to the parent bank ratio of 1:1; Seedlings 4000.
13. A Breeding Method for Resisting Picking Chinese Cabbage
Application (Patent) No. 0
Abstract: The present invention discloses a breeding method for anti-convulsive Chinese cabbage, the mother-selected anti-convulsant self-incompatible self-incompatibility germplasm material 01S315 of the anti-convulsive Chinese cabbage, and the parent-selected convulsive self-intergrity germplasm. Material 01S105, female parent and male parent crossbreed, the anti-convulsive Chinese cabbage selected by the method of the present invention is a mid-maturity hybrid, and the growth period is about 60-65 days (from planting to harvesting). Plant height 37.1cm, degree of development 60.3cm, strong growth potential, 13 outer leaves, dark green leaves, petiole white. The leaves are hung together, obovate, and the ball tight. The ball leaves are white, the ball height is 28.3cm, the ball width is 19.6cm, the ball index is 1.5, the single ball weight is 1.8-2.5kg, the net vegetable rate is 73.9%. The number of spherules is about 60 pieces, and the resistance to priming is extremely strong. When the seeds are planted, the parental flowering period is 7-10 days earlier than the male parent, and the father has a stronger winter nature. Therefore, the male parent must be sowed 15 to 25 days earlier than the female parent. The ratio of mother to father is 2:1; 4000 to 4,500 mu are left.